Researcher placing a reaction tube in the Quantitative Real-Time Polymerase Chain Reaction Instrument

Researcher placing a reaction tube in the Quantitative Real-Time Polymerase Chain Reaction Instrument

In the inset, the lines on this graph show how different expression levels can be detected by this assay. Each line on this graph is for a single reaction. The further a reaction is shifted to the left, the higher the expression level of the gene of interest in comparison to the other reactions.